Vol II The COLOSS BEEBOOK, Vol II Standard methods for Apis mellifera pest and pathogen research ¿ Vincent Dietemann, James D. Ellis, & Peter Neumann (Eds) The COLOSS (Prevention of honey bee COlony LOSSes) BEEBOOK is a unique venture that aims to standardise methods for studying the honey bee. It is a practical manual intended for scientists, extension specialists and beekeepers, compiling standard methods in all fields of research on the honey bee, Apis mellifera. The first two volumes are composed of 32 peer-reviewed chapters authored by more than 234 of the world's leading honey bee experts from 34 different countries. Since their original publication in 2013, the BEEBOOK has become the definitive bee research manual, and the original papers have been downloaded some 180,000 times and cited in more than 2,300 scientific papers. The hard copy volumes have proved invaluable at the laboratory bench. Volume II includes approximately 600 separate protocols dealing with the study of the pests and diseases of the honey bee, Apis mellifera. These cover epidemiology and surveying techniques, virus diseases, bacterial diseases such as European and American foulbrood, fungal and microsporidian diseases such as Nosema, mites such as Acarapis, Varroa and Tropilaelaps, and other pests such as the small hive beetle.